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AIM: To establish an experimental model of high intraocular pressure in mice by laser photocoagulation and to prepare for future research. METHODS: Experimental model of high intraocular pressure was induced unilaterally in 44 C57BL/6 mice. The fellow eye served as a control. TONO-PEN AVIA Tonomter was used to measure intraocular pressure (IOP) to guarantee IOP value at 1, 2, 4, 8wk. Slit-lamp biomicroscopy was performed throughout the period and the structural changes were assessed histologically. And then, their eyes were enucleated, postfixed, cryoprotected, and embedded in optimal cutting temperature medium. After hematoxylin and eosin stain ( HE stain ) , cryosections of the retina were observed under light microscope. TdT-mediated biotin-dUTP nick end labeling ( TUNEL ) was performed on the retinal sections to determine apoptosis rate. RESULTS: IOP of laser-treated eyes was significantly higher than that of control eyes from 1-8wk (P CONCLUSION: The laser photocoagulation of limbus causes chronic elevation of IOP and this method may be a promising experimental model for the investigation of biological mechanisms of glaucomatous retinal ganglion cell damage.
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<p><b>OBJECTIVE</b>To study the therapeutic effect of electroacupuncture on patients with osteoarthritis, and to observe the effect of electroacupuncture on the level of IL-1beta and TNFalpha.</p><p><b>METHODS</b>Forty-three subjects received electroacupuncture treatment at Liangqiu (ST 34), Xuehai (SP 10), Yinlingquan (SP 9), etc. The intensity of pain, the ability of the movement of legs, the level of IL-1beta and TNF-alpha in patients were measured before and after treatment.</p><p><b>RESULTS</b>According to VAS, the intensity of pain was (64.22 +/- 14.55) and (32.50 +/- 10.79) before and after treatment, respectively. The score of movement ability of affected legs was (7.38 +/- 2.01) and (9.05 +/- 1.89) before and after treatment, respectively. Thus, the intensity of pain and the ability of movement were significantly improved after treatment (P < 0.01). The level of IL-1beta and TNF-alpha in patients were significantly decreased (both P < 0.05).</p><p><b>CONCLUSION</b>The short term therapeutic effect of electroacupuncture on patients with osteoarthritis is better, it may result from that the electroacupuncture inhibit the activity of IL-1beta and TNF-alpha.</p>
Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Electroacupuncture , Interleukin-1beta , Blood , Osteoarthritis , Blood , Therapeutics , Tumor Necrosis Factor-alpha , BloodABSTRACT
@#ObjectiveTo investigate the effect of nifedipine(Nif) on experimental pulmonary hypertension(PH).Methods33 adult male Wistar rats were randomly divided into normal control group,model group and Nif treatment group(n=11 for each group). Rats were given a single dose of subcutaneous monocrotaline(MCT,50 mg/kg) to induce the model of pulmonary hypertension, then treated with gastric infusion of normal saline or Nif respectively for 21 days. The parameters of pulmonary hemodynamics were monitored by Gould3400 polygraph system through the cannulation of polyvinyl tube. Right ventricle and left ventricle added with interventricular septum weight ratio, and the lung wet weight were measured.ResultsNif significantly inhibited the progression of pulmonary artery pressure(P<0.01), decreased the right heart index(P<0.01), but did not significantly reduce the lung wet weight(P>0.05).ConclusionChronic treatment with Nif may effectively reduce the development of PH induced by MCT and improve the heart function.